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-acetyl derivative. This result is in line with the formation or collapse of the tetrahedral intermediate as the rate restricting move inside the catalytic mechanism of NagA.
Web page certain mutants of NagA had been produced so as to confirm the roles of particular residues in substrate recognition and catalytic functionality. The metallic content material plus the kinetic parameters with the mutants of NagA manufactured for this investigation are introduced in Desk 4.
The exact role of your metal cofactor is of certain fascination for NagA as the two crystal buildings readily available for the enzyme from E. coli
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Mutation of H143 to an asparagine resulted inside the spectacular loss of catalytic action along with a average increase in slot naga26 the K
cat vs. pH-profile shown in Determine 2A for Zn-NagA was fit to equation 3 and these final results reveal which the deprotonation of one acid using a kinetic p
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